pEASY?-Uni Seamless Cloning and Assembly Kit
通用版同源重組無(wú)縫克隆試劑盒
| 目錄號(hào) | 規(guī)格 | 單價(jià) |
|---|---|---|
| CU101-01 | 10 rxns | 1000 |
| CU101-02 | 20 rxns | 1800 |
| CU101-03 | 60 rxns | 4330 |
產(chǎn)品詳情介紹
本產(chǎn)品利用特殊的重組酶和同源重組的原理，可以將任意方法線性化后的載體和與其兩端具有15-25 bp重疊區(qū)域的PCR片段
定向重組，可以實(shí)現(xiàn)最多15個(gè)片段的高效無(wú)縫拼接。
? 快速：僅需要5~15分鐘反應(yīng)時(shí)間。
? 簡(jiǎn)單：不受片段酶切位點(diǎn)的影響，無(wú)需對(duì)片段酶切。
? 高效：陽(yáng)性率達(dá)95% 以上。
? 無(wú)縫：不引入額外的序列。
組裝效率高
14 kb 長(zhǎng)載體組裝
低濃度多片段成功連接(片段投入量為常規(guī)投入量的 1/10)
1.Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)
2.Shi J, Mei C, Ge F, et al. Resistance to Striga parasitism through reduction of strigolactone exudation[J]. Cell, 2025.(IF 42.50)
3.Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)
4.Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 21.10)
5.Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024.(IF 17.30)
6.Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 14.92)
7.Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 14.90)
8.Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)
9.Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)
10.Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)
11.Mu S, Chen H, Li Q, et al. Enhancing prime editor flexibility with coiled-coil heterodimers[J]. Genome Biology, 2024,(IF 10.10)
12.Du G, Xiong L, Li X, et al. Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair[J]. Developmental cell, 2020,(IF 10.09)
13.Tang Y, Gao C C, Gao Y, et al. OsNSUN2-mediated 5-methylcytosine mRNA modification enhances rice adaptation to high temperature[J]. Developmental cell, 2020.(IF 10.09)
14.Li J, Bai Y, Liu Y, et al. Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems[J]. Cell Reports, 2023.(IF 8.80)
15.Que Y, Shu X, Wang L, et al. Inactivation of SERCA2 Cys674 accelerates aortic aneurysms by suppressing PPARγ[J]. British Journal of Pharmacology, 2021,(IF 8.74)
16.Wang Y, Dong H, Qu Y, et al. Circabidian rhythm of sex pheromone reception in a scarab beetle[J]. Current Biology, 2024,(IF 8.10)
17.Sun S, Liu D, Luo W, et al. Domestication-selected COG4-OsbZIP23 module regulates chilling tolerance in rice[J]. Cell Reports, 2024,(IF 7.50)
18.Du J, Huang Z, Li Y, et al. Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly proteins[J]. Free Radical Biology and Medicine, 2023.(IF 7.40)
19.Zhu L, Li B, Li R, et al. METTL3 suppresses pancreatic ductal adenocarcinoma progression through activating endogenous dsRNA-induced anti-tumor immunity[J]. Cellular Oncology, 2023.(IF 6.60)
20.Song N, Chen L, Ren X, et al. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells[J]. PLoS Pathogens, 2021.(IF 6.20)
21.Liu S, Zhang M, Bao Y, et al. Characterization of a Highly Selective 2 ″-O-Galactosyltransferase from Trollius chinensis and structure-guided engineering for improving UDP-glucose selectivity[J]. Organic Letters, 2021.(IF 6.00)
22.Tao Y, Yang Y, Yang Z, et al. Robust small molecule-aided cardiac reprogramming systems selective to cardiac fibroblasts[J]. IScience, 2023,(IF 5.80)
23.Duan Y, Liu S, Gao Y, et al. Macrolides mediate transcriptional activation of the msr (E)-mph (E) operon through histone-like nucleoid-structuring protein (HNS) and cAMP receptor protein (CRP)[J]. Journal of Antimicrobial Chemotherapy, 2022.(IF 5.80)
24.Hu M, Li Z, Li D, et al. Long Terminal Repeat Retrotransposon Afut4 promotes azole resistance of Aspergillus fumigatus by enhancing the expression of sac1 gene[J]. Antimicrobial Agents and Chemotherapy, 2021,(IF 5.19)
25.Zheng M, Jiang Y, Ran Z, et al. A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress[J]. Communications Biology, 2025.(IF 5.10)
